Analysis of enzyme kinetics by using integrated rate equations. Arginine decarboxylase
نویسندگان
چکیده
منابع مشابه
Progress-curve analysis in enzyme kinetics. Numerical solution of integrated rate equations.
Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes direct calculation of the extent of reaction at any time. Previously, such equations have been solved by the Newton-Raphson method, but this procedure may fail when based upon the usual formulae. An alternative formulation is proposed that is both quicker and more robust.
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15 صفحه اولThe Kinetics of Enzyme Mixtures
Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme. For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present. Based...
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متن کاملUse of integrated rate equations in estimating the kinetic constants of enzyme-catalyzed reactions.
Methods are described for estimation of kinetic constants from the integrated rate equations for a number of reaction mechanisms. In general, these ( methods depend upon elimination of either zero order or fist order terms between rate curves determined at two initial substrate concentrations or more. This operation is followed by graphic or statistical evaluation of the kinetic constants in th...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2450059